Fluorescent labeling and confocal microscopic imaging of chloroplasts and non-green plastids.
Identifieur interne : 000408 ( Main/Exploration ); précédent : 000407; suivant : 000409Fluorescent labeling and confocal microscopic imaging of chloroplasts and non-green plastids.
Auteurs : Maureen R. Hanson [États-Unis] ; Amirali SattarzadehSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2014.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Chlorophylle (métabolisme), Chloroplastes (génétique), Coloration et marquage (méthodes), Microscopie confocale (méthodes), Microscopie de fluorescence (méthodes), Protéines bactériennes (génétique), Protéines luminescentes (génétique), Protéines végétales (métabolisme), Protéines à fluorescence verte (génétique), Tabac (génétique), Transformation génétique (MeSH), Transgènes (génétique), Végétaux génétiquement modifiés (génétique).
- MESH :
- génétique : Agrobacterium tumefaciens, Chloroplastes, Protéines bactériennes, Protéines luminescentes, Protéines à fluorescence verte, Tabac, Transgènes, Végétaux génétiquement modifiés.
- métabolisme : Chlorophylle, Protéines végétales.
- méthodes : Coloration et marquage, Microscopie confocale, Microscopie de fluorescence.
- Transformation génétique.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Bacterial Proteins (genetics), Chlorophyll (metabolism), Chloroplasts (genetics), Green Fluorescent Proteins (genetics), Luminescent Proteins (genetics), Microscopy, Confocal (methods), Microscopy, Fluorescence (methods), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Staining and Labeling (methods), Tobacco (genetics), Transformation, Genetic (MeSH), Transgenes (genetics).
- MESH :
- chemical , genetics : Bacterial Proteins, Green Fluorescent Proteins, Luminescent Proteins.
- genetics : Agrobacterium tumefaciens, Chloroplasts, Plants, Genetically Modified, Tobacco, Transgenes.
- chemical , metabolism : Chlorophyll, Plant Proteins.
- methods : Microscopy, Confocal, Microscopy, Fluorescence, Staining and Labeling.
- Transformation, Genetic.
Abstract
While chlorophyll has served as an excellent label for plastids in green tissue, the development of fluorescent proteins has allowed their ready visualization in all tissues of the plants, revealing new features of their morphology and motility. Gene regulatory sequences in plastid transgenes can be optimized through the use of fluorescent protein reporters. Fluorescent labeling of plastids simultaneously with other subcellular locations reveals dynamic interactions and mutant phenotypes. Transient expression of fluorescent protein fusions is particularly valuable to determine whether or not a protein of unknown function is targeted to the plastid. Particle bombardment and agroinfiltration methods described here are convenient for imaging fluorescent proteins in plant organelles. With proper selection of fluorophores for labeling the components of the plant cell, confocal microscopy can produce extremely informative images at high resolution at depths not feasible by standard epifluorescence microscopy.
DOI: 10.1007/978-1-62703-995-6_7
PubMed: 24599850
Affiliations:
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Gene regulatory sequences in plastid transgenes can be optimized through the use of fluorescent protein reporters. Fluorescent labeling of plastids simultaneously with other subcellular locations reveals dynamic interactions and mutant phenotypes. Transient expression of fluorescent protein fusions is particularly valuable to determine whether or not a protein of unknown function is targeted to the plastid. Particle bombardment and agroinfiltration methods described here are convenient for imaging fluorescent proteins in plant organelles. With proper selection of fluorophores for labeling the components of the plant cell, confocal microscopy can produce extremely informative images at high resolution at depths not feasible by standard epifluorescence microscopy. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24599850</PMID><DateCompleted><Year>2014</Year><Month>11</Month><Day>18</Day></DateCompleted><DateRevised><Year>2014</Year><Month>03</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1132</Volume><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Fluorescent labeling and confocal microscopic imaging of chloroplasts and non-green plastids.</ArticleTitle><Pagination><MedlinePgn>125-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-62703-995-6_7</ELocationID><Abstract><AbstractText>While chlorophyll has served as an excellent label for plastids in green tissue, the development of fluorescent proteins has allowed their ready visualization in all tissues of the plants, revealing new features of their morphology and motility. Gene regulatory sequences in plastid transgenes can be optimized through the use of fluorescent protein reporters. Fluorescent labeling of plastids simultaneously with other subcellular locations reveals dynamic interactions and mutant phenotypes. Transient expression of fluorescent protein fusions is particularly valuable to determine whether or not a protein of unknown function is targeted to the plastid. Particle bombardment and agroinfiltration methods described here are convenient for imaging fluorescent proteins in plant organelles. 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